(NaturalNews) Making natural products is simple and inexpensive. In every first aid kit be sure to include a balm for bumps, bruises, and sprains. The best known herb for this is Arnica. Arnica has been used as medicine for hundreds of years. It has anti-inflammatory and antimicrobial…

Source: NaturalNews.comCategory: Consumer Health News Source Type: news

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Publication date: Available online 9 August 2018Source: PathophysiologyAuthor(s): Nahuel A. Ramella, Isabel Andújar, José L. Ríos, Silvana A. Rosú, M. Alejandra Tricerri, Guillermo R. SchinellaAbstractThe cascade of molecular events leading to Human apolipoprotein A-I (apoA-I) amyloidosis is not completely understood, not even the pathways that determine clinical manifestations associated to systemic protein deposition in organs such as liver, kidney and heart. About twenty natural variants of apoA-I were described as inducing amyloidosis, but the mechanisms driving their aggregation and deposit…

Source: PathophysiologyCategory: Pathology Source Type: research

This study proposes a novel approach for the detection of hemoglobin in animal feed by synchronous fluorescence spectroscopy (SFS). The objective was to develop a fast and easy method to detect hemoglobin powder and blood meal. Analyses were carried out on standard reference material (hemoglobin and albumin) in order to optimize SFS method conditions for hemoglobin detection. The method was then applied to protein extracts of commercial feed material and compound feed. The results showed that SFS spectra of blood meal and blood products (hemoglobin powder and plasma powder) could be used to characterize hemoglobin. Princip…

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research

Abstract
A facile and sensitive photoelectrochemical (PEC) sensing platform based on zinc phthalocyanine (ZnPc) sensitized TiO2 nanorodarrays (NRAs) was successfully fabricated for detection of bisphenol A (BPA). The vertically aligned TiO2 NRAs with one-dimensional (1D) nanostructure was synthesized in-situ on fluorine-doped tin oxide-coated glass (FTO) substrate by simple one-step hydrothermal method, and then the TiO2 NRAs/FTO surface was covered with ZnPc. 1D nanostructure of TiO2 NRAs could provide not only more spaces for the load of ZnPc, but direct pathways to reduce the recombination of electron-hole pair…

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research

Abstract
A possibility of harvesting the physiologically-active compounds from the vegetable oils using bespoke resin and optimised solid-phase extraction (SPE) purification method is demonstrated. The paper describes the application of SPE protocol, which was originally developed, using sunflower oil as model biomass, for extraction of the valuable compounds from sesame, wheat germ, palm, olive and soybean oils. As a result, the extraction of three free fatty acids (palmitic, oleic and linoleic), α-tocopherol and three phytosterols (campesterol, stigmasterol and β-sitosterol) from six vegetable oils ha…

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research

Abstract
A pyrene-based colorimetric and fluorescent pH probe with large Stokes shift (145 nm) was developed. PNY afforded a pKa of 2.98 and responded linearly to minor pH changes within the range of 2.43 and 3.71. In addition, PNY could be used for detecting H+ with high sensitivity and selectivity, showing colorimetric and fluorometric dual-modal responses with short response time. Furthermore, PNY had good photostability, excellent reversibility and cell membrane permeability. Significantly, PNY could image acidic pH in A549 cells and visualized extreme acidity in E. coli cells.
PMID: 30086899 [PubMed – in process]

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research

Magnetic nanoparticles and polydopamine amplified FP aptasensor for the highly sensitive detection of rHuEPO-α.
Talanta. 2018 Nov 01;189:143-149
Authors: Chen Z, Li H, Zhao Y, Xu M, Xu D
Abstract
In this paper, an amplified fluorescence polarization (FP) aptasensor based on magnetic nanoparticles @polydopamine ([email protected]) was innovatively developed for sensitive detection of recombinant human erythropoietin-alpha(rHuEPO-α). The amplified FP signal was due to the large mass of protein and [email protected] And this assay can be utilized for target separation or recycling based on the magnetic property…

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research

Abstract
A label-free light-up fluorescent sensing strategy using hybridization chain reaction (HCR) amplification and DNA triplex assembly has been developed. Remarkably, the proposed fluorescence assay is successfully applied to the determination of avian influenza A (H7N9) virus DNA and thrombin. Herein, in the presence of targets, the target DNA/initiator triggers a cascade of hybridization events between H1 and H2 that yields nicked double helices analogous to alternating copolymers. With the additions of triplex-forming oligonucleotide (TFO) and berberine, the triplex structures form between HCR products and…

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research

Abstract
A highly sensitive colorimetric method for detection of alkaline phosphatase (ALP) and carbohydrate antigen (CA125) was developed, which was achieved by ascorbic acid (AA) – mediated enhanced growth of silver nanoparticles (AgNPs) promoted with NaBH4 as pre-reducing agent. With prereduction by NaBH4, the AA controlled growth of AgNPs was promoted and the localized surface plasmon resonance absorbance was much higher compared with that without prereduction. ALP could hydrolyze L-ascorbic acid 2-phosphate trisodium salt (AAP) to form AA, which could then reduce Ag+ to AgNPs resulting in the enhancement of l…

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research

Abstract
Highly sensitive detection of proteins is essential to biomedical research as well as clinical diagnosis. However, usual detecting methods are complicated operating, time-costing and extensive label preparing. Here, we combined molecular beacon (MB) track mediated DNA walker and nicking enzyme assisted signal amplification method to develop a simple and ultrasensitive malachite green fluorescence biosensor for specific detection cytokine, interferon-γ (IFN-γ). The association of the IFN-γ with the corresponding aptamers of the dsDNA strands leads to free of DNA walker which trigged the g…

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research

In this study, a novel air-assisted liquid-liquid microextraction (AALLME) method was developed for the HPLC determination of bisphenols migration from disposable plastic lunch box to contacting water. The AALLME was carried out in commercially-available medical plastic syringes with low-density extraction solvent (n-octanol), and the reaction of NaHCO3 and HCl was introduced to simultaneously induce the in-situ phase separation and salt effect. After the AALLME processes, the upper organic phase was pushed into the end of syringes (narrow tube) by moving the piston so that it could be readily collected for the HPLC analys…

Source: TalantaCategory: Chemistry Authors: Tags: Talanta Source Type: research





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